trisedta buffer (Thermo Fisher)

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Thermo Fisher trisedta buffer
Trisedta Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trisedta+buffer/TRIS+EDTA+BUFFER/pm42032366-66-14-22
Average 97 stars, based on 1 article reviews

trisedta buffer - by Bioz Stars, 2026-09

97/100 stars

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Polymerase Chain Reaction:

Article Title: Highly Sensitive Molecular Assay for the Detection of Treponema pallidum infection
Article Snippet: 3.2 T. pallidum Genomic DNA Preparation T. pallidum DNA was obtained from the American Type Culture Collection (ATCC) (ATCC BAA 2642SD) at a concentration of ≥ 1 x 105 copies/μL. .. T. pallidum genomic DNA was diluted from 8.14×104 to 8.14×10− 2 genome copies/μL and used as template for both IMRS and 16S rRNA PCR assays in TrisEDTA buffer (Thermo Fisher Scienti c, Waltham, Massachusetts, USA). .. 3.3 16S rRNA PCR assay The 16S rRNA PCR assays were carried out in a reaction mixture containing dNTPs (Thermo Fisher Scienti c, Waltham, Massachusetts, USA) (0.2 mM), forward and reverse primers (0.01 mM each), Taq Hot-Start DNA polymerase (Thermo Fisher Scienti c, Waltham, Massachusetts, USA) (1.25 U), genomic template DNA (1 μL to a nal PCR reaction volume of 25 μL).

Gene Expression:

Article Title: When translation elongation is impaired, the mRNA is uniformly destabilized by the RNA degradosome, while the concentration of mRNA is altered along the molecule.
Article Snippet: .. The pre-amplified samples were treated with 8 U of exonuclease I (New England BioLabs), diluted 5-fold with TrisEDTA buffer and added to a ‘Sample Mix’ consisting of TaqMan® Gene expression Master Mix (Applied Biosystems), DNA Binding Dye Sample Loading Reagent (Fluidigm), EvaGreen® dye (Biotium) plus Tris-EDTA buffer, as recommended. .. In parallel, each primer pair (20 M) was added to a ‘Primer Mix’ composed of assay loading reagent (Fluidigm) plus Tris-EDTA buffer, as recommended.

Binding Assay:

Incubation:

Article Title: The microbiome modulating potential of superheated steam (SHS) treatment of dietary fibres
Article Snippet: .. The pellet was then resuspended in 100 μL TrisEDTA buffer and 2 μL RNase (10 mg/mL; Thermofisher, MA, USA) was added and incubated at 37 ◦C for 15 min. .. Following incubation, 15 μL proteinase K (QIAmp DNA Stool Mini Kit, Qiagen, Germany) and 200 μL AL buffer (QIAmp DNA Stool Mini Kit, Qiagen, Germany) were added to samples and incubated at 70 ◦C for 10 min. 200 μL 100% ethanol was added to samples for precipitation.

Immunohistochemistry:

Article Title: Combination therapy with Chicoric acid and PD-1/PD-L1 blockade improves the immunotherapy response in patient-derived ovarian cancer xenograft model.
Article Snippet: .. For IHC, antigen retrieval was performed using TrisEDTA buffer (95 °C, 20 min), and the samples were cooled in a water bath for 5 min. Rabbit anti-human CD8 alpha (ab205921, 1:1000 dilution; Abcam, Cambridge, UK) or rabbit anti-human PD-L1 (PA5-28115, 1:800 dilution; Invitrogen, USA) was added. .. The next steps were performed via the Mouse and Rabbit Specific HRP/DAB IHC Detection Kit-Micropolymer (ab236466; Abcam, Cambridge, UK) according to the manufacturer’s instructions.

Concentration Assay:

Article Title: Evaluation of the diagnostic accuracy of Xpert® Mpox and STANDARD™ M10 MPX/OPX for the detection of monkeypox virus.
Article Snippet: .. Synthetic DNA (Eurofins Genomics, UK) was re-hydrated in TrisEDTA buffer and concentration was quantified using QubitTM SSDNA Quantification Assay kit (ThermoFisher, USA). ..

Control:

Article Title: Detection of the residual concentration of sodium dodecyl sulfate in the decellularized whole rabbit kidney extracellular matrix.
Article Snippet: To optimize rabbit kidney decellularization protocol, using sodium dodecyl sulfate (SDS) as a commonly used detergent, a methylene blue based assay was employed for detecting the minimum nontoxic SDS level for future cell seeding.. The rabbit kidney tissues were decellularized with the perfusionbased method and underwent several investigations to determine the efficacy of decellularization in preserving the extracellular matrix (ECM) and cell removal.. SDS detection was performed by incubating with methylene blue and subsequent extraction with chloroform.

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